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Image Search Results
Journal: Virology Journal
Article Title: Glucose-induced active lysogeny of prophage ΦSa3XN promotes Staphylococcus aureus virulence
doi: 10.1186/s12985-025-02993-3
Figure Lengend Snippet: ΦSa3XN excision-restored Hlb promotes S. aureus virulence in hyperglycemic mice. ( A ) The relative excision rates of ΦSa3XN from overnight cultures of XN108 and Δ hlb C that untreated or supplemented with 2% glucose were determined by qPCR. The ΦSa3XN excision rate for untreated XN108 was set to 1. Data are the mean ± SD from three independent replicates and analyzed by two-way ANOVA with Sidak’s multiple comparisons test, ** P < 0.01; ns, not significant. ( B ) Hemolytic activities of XN108 and Δ hlb C as treated in ( A ). The hemolytic activity of untreated XN108 was set to 1. Data are the mean ± SD from three independent replicates and determined by two-way ANOVA with Sidak’s multiple comparisons test, **** P < 0.0001; ns, not significant. ( C ) Schematic illustrating the animal experimental procedure. ( D ) The 6-h fasting blood glucose levels of mice before and after streptozotocin injection. Five mice successfully developed hyperglycemia with FBG more than 11.1 mmol/L (the dotted line). ( E ) Monitoring for abscess formation. Data are the mean ± SD ( n = 5 mice) and analyzed by two-way ANOVA with Sidak’s multiple comparisons test, * P < 0.05. ( F ) Representative images of mouse skin damage. ( G ) Representative HE staining of mouse skin tissues collected on day 6 post-infection. The red triangles indicate the infiltration of inflammatory cells, black triangles show the vascular congestion, and blue triangles point to the loosed arrangement of connective tissues. ( H ) The severity of the skin lesions in histological sections of ( G ) was numerically classified with ImageJ software, and the relative inflammatory cell infiltration area (%) was calculated and indicated. Three sections with the bars of 200 μm were used. Statistical significance was determined by Student’s t -test, * P < 0.05
Article Snippet:
Techniques: Activity Assay, Injection, Staining, Infection, Software